Recombinant HSP90 Rabbit Monoclonal Antibody

Immunofluorescent analysis of (4% PFA) fixed Hela cellusing BS5014(HSP90 antibody) at dilution of 1:50 (under400xlens)
Western blot analysis of HSP90 on different lysates.Proteins were transferred to a NC membrane and blockedwith 5% NF-Milk in TBST for 1 hour at roomtemperature. The primary antibody (BS5014, 1/1000)was used in TBST at room temperature for 2 hours.HRP-Goat Anti-Rabbit Secondary Antibody(H+L)(BS1101) at 1:5,000 dilution was used for 1 hour at roomtemperature.Lane 1: HEK293 cell Lane 2: Hela cell Lane3: MCF-7 cell Lane 4: SIHa cell Lane 5: RT4 cell Lane 6:COS7 cell Lane 7:3D4/21 cell
Immunohistochemical analysis of paraffin embedded ratTestis tissue slide using BS5014(HSP90 antibody) atdilution of 1:200 (under 400x lens). Heat mediatedantigen retrieval with Sodium citrate buffer (pH 6.0)
Immunohistochemical analysis of paraffin embeddedmouse Testis tissue slide using ZH5014(HSP90 antibody)at dilution of 1:200 (under 400x lens). Heat mediatedantigen retrieval with Sodium citrate buffer (pH 6.0)

货号：BS5014

Recombinant HSP90 Rabbit Monoclonal Antibody

Heat shock 86 kDa; Heat shock protein 90kDa alpha cytosolic class A member 1; Heat shock protein 90kDa alpha cytosolic class B member 1; Heat shock protein HSP 90 alpha; Heat shock protein HSP 90 beta; Heat shock protein HSP 90-alpha; HS90A; HSP 84; HSP 86; HSP86; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSPC1; HSPC2; HSPCAL1; HSPCAL4; Renal carcinoma antigen NY-REN-38; Hsp 90; HSP90

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别名	Heat shock 86 kDa; Heat shock protein 90kDa alpha cytosolic class A member 1; Heat shock protein 90kDa alpha cytosolic class B member 1; Heat shock protein HSP 90 alpha; Heat shock protein HSP 90 beta; Heat shock protein HSP 90-alpha; HS90A; HSP 84; HSP 86; HSP86; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSPC1; HSPC2; HSPCAL1; HSPCAL4; Renal carcinoma antigen NY-REN-38; Hsp 90; HSP90
推荐应用
反应物种	Human;Mouse;Rat;Monkey;Pig
存储缓冲液	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
基因ID	3320/3326 (Human),15519/15516 (Mouse),299331/301252 (Rat)
免疫原	Recombinant fragment within Human Hsp90 alpha+beta. AA range: 500 to the C-terminus.
稀释度	WB 1:500-1:2000;IHC-P 1:100-1:500;ICC/IF 1:50-1:200;IF-P:100-1:500;IF-Fr:100-1:500;IP 1:100-1:300;FC 1:50-1:200;
参考分子量	90 kDa
预测分子量	85 kDa
运输及保存条件	Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.
宿主	Rabbit
Uniprot ID	P08238;P11499;P34058;P11499;P82995;P34058;

Recombinant HSP90 Rabbit Monoclonal Antibody

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