| 别名 | Hormone-sensitive lipase |
| 基因名称 | Endogenous |
| 蛋白名称 | LIPE |
| 推荐应用 | |
| 反应物种 | HSL |
| 存储缓冲液 | WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | LIPE;Hormone-sensitive lipase;HSL |
| Human Swissprot No. | 3991 |
| Mouse Gene ID | Q05469 |
| Mouse Swissprot No. | 16890 |
| Rat Gene ID | 25330 |
| Rat Swissprot No. | P15304 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 117kD |
| 预测分子量 | WB,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 81kD |
| 同种型 | Rabbit |
| 背景介绍 | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008], |
| 细胞定位 | Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). . |
| 功能 | Catalytic activity:Diacylglycerol + H(2)O = monoacylglycerol + a carboxylate.,Catalytic activity:Monoacylglycerol + H(2)O = glycerol + a carboxylate.,Catalytic activity:Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.,enzyme regulation:Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.,Function:In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.,pathway:Glycerolipid metabolism; triacylglycerol degradation.,similarity:Belongs to the 'GDXG' lipolytic enzyme family.,subcellular location:Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.,subunit:Interacts with PTRF in the adipocyte cytoplasm., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). . |
| 组织表达 | Testis. |
| 研究领域 | >>cAMP signaling pathway;>>AMPK signaling pathway;>>Apelin signaling pathway;>>Thermogenesis;>>Insulin signaling pathway;>>Regulation of lipolysis in adipocytes;>>Aldosterone synthesis and secretion |
周一至周日 8:00-18:00
