| 别名 | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (O-GlcNAc transferase subunit p110) (O-linked N-acetylglucosamine transferase 110 kDa subunit) (OGT) |
| 基因名称 | Endogenous |
| 蛋白名称 | OGT |
| 推荐应用 | |
| 反应物种 | OGT |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000; |
| Human Gene Link | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit;O-GlcNAc transferase subunit p110;O-linked N-acetylglucosamine transferase 110 kDa subunit;OGT; |
| Human Swissprot No. | 8473 |
| Mouse Gene ID | O15294 |
| Mouse Swissprot No. | 108155 |
| Rat Gene ID | 26295 |
| Rat Swissprot No. | P56558 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 117kD |
| 预测分子量 | WB,IHC,IF,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 117kD |
| 同种型 | Rabbit |
| 背景介绍 | This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009], |
| 细胞定位 | Nucleus . Cytoplasm . Predominantly localizes to the nucleus. .; [Isoform 2]: Mitochondrion . Membrane . Associates with the mitochondrial inner membrane. .; [Isoform 3]: Cytoplasm . Nucleus . Cell membrane . Mitochondrion membrane . Cell projection . Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1 (PubMed:21285374). After insulin induction, translocated from the nucleus to the cell membrane via phosphatidylinositide binding. Colocalizes with AKT1 at the plasma membrane. TRAK1 recruits this protein to mitochondria. In the absence of TRAK1, localizes in cytosol and nucleus (By similarity). .; [Isoform 4]: Cytoplasm. Nucleus. |
| 功能 | Catalytic activity:UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.,Function:Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine.,online information:UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110kDa subunit,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the O-GlcNAc transferase family.,similarity:Contains 13 TPR repeats.,subunit:Heterotrimer of two 110 kDa and one 70 kDa subunits. It is not known if the 70 kDa subunit is encoded by a separate gene or is the product of either of a proteolytic degradation or an alternative initiation of the 110 kDa subunit (By similarity). Interacts with HCFC1.,tissue specificity:Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Nucleus . Cytoplasm . Predominantly localizes to the nucleus. .; [Isoform 2]: Mitochondrion . Membrane . Associates with the mitochondrial inner membrane. .; [Isoform 3]: Cytoplasm . Nucleus . Cell membrane . Mitochondrion membrane . Cell projection . Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1 (PubMed:21285374). After insulin induction, translocated from the nucleus to the cell membrane via phosphatidylinositide binding. Colocalizes with AKT1 at the plasma membrane. TRAK1 recruits this protein to mitochondria. In the absence of TRAK1, localizes in cytosol and nucleus (By similarity). .; [Isoform 4]: Cytoplasm. Nucleus. |
| 组织表达 | Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. |
| 研究领域 | >>Other types of O-glycan biosynthesis;>>Insulin resistance |
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