| 别名 | Serine/arginine-rich splicing factor 1 (Alternative-splicing factor 1) (ASF-1) (Splicing factor, arginine/serine-rich 1) (pre-mRNA-splicing factor SF2, P33 subunit) |
| 基因名称 | Endogenous |
| 蛋白名称 | SRSF1 ASF SF2 SF2P33 SFRS1 OK/SW-cl.3 |
| 推荐应用 | |
| 反应物种 | SRSF1 |
| 存储缓冲液 | IHC 1:500-1:2000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000; |
| Human Swissprot No. | 6426 |
| Mouse Gene ID | Q07955 |
| Mouse Swissprot No. | 110809 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 28kD |
| 预测分子量 | WB,IHC,IF,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 28kD |
| 同种型 | Rabbit |
| 细胞定位 | Cytoplasm . Nucleus speckle . In nuclear speckles. Shuttles between the nucleus and the cytoplasm (PubMed:12215544, PubMed:20308322, PubMed:9420331, PubMed:24449914). Nuclear import is mediated via interaction with TNPO3 (PubMed:24449914). . |
| 功能 | Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cytoplasm . Nucleus speckle . In nuclear speckles. Shuttles between the nucleus and the cytoplasm (PubMed:12215544, PubMed:20308322, PubMed:9420331, PubMed:24449914). Nuclear import is mediated via interaction with TNPO3 (PubMed:24449914). . |
周一至周日 8:00-18:00
