| 别名 | Heterogeneous nuclear ribonucleoprotein K |
| 基因名称 | Endogenous |
| 蛋白名称 | HNRNPK |
| 推荐应用 | |
| 反应物种 | hnRNP K |
| 存储缓冲液 | IHC 1:1000-1:4000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | HNRNPK;HNRPK;Heterogeneous nuclear ribonucleoprotein K;hnRNP K;Transformation up-regulated nuclear protein;TUNP |
| Human Swissprot No. | 3190 |
| Mouse Gene ID | P61978 |
| Mouse Swissprot No. | 15387 |
| Rat Gene ID | 117282 |
| Rat Swissprot No. | P61980 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 51kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 62kD |
| 同种型 | Rabbit |
| 背景介绍 | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have |
| 细胞定位 | Cytoplasm . Nucleus, nucleoplasm . Cell projection, podosome . Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702). |
| 功能 | Function:One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.,mass spectrometry: PubMed:11840567,PTM:Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.,similarity:Contains 1 KH domain.,similarity:Contains 2 KH domains.,similarity:Contains 3 KH domains.,subcellular location:In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.,subunit:Interacts with RBM42 and ZIK1 (By similarity). Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRNPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with ANKRD28. Interacts with HCV core protein. Interacts with ASFV p30 protein., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cytoplasm . Nucleus, nucleoplasm . Cell projection, podosome . Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702). |
| 组织表达 | Brain,Cajal-Retzius cell,Colorectal cancer and surrounding |
| 研究领域 | >>Spliceosome;>>Viral carcinogenesis;>>MicroRNAs in cancer |
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