| 别名 | Pancreas/duodenum homeobox protein 1 |
| 基因名称 | Endogenous |
| 蛋白名称 | PDX1 |
| 推荐应用 | |
| 反应物种 | PDX1 |
| 存储缓冲液 | IHC 1:100-1:500;WB 1:500-1:20000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | PDX1;IPF1;Pancreas/duodenum homeobox protein 1;PDX-1;Glucose-sensitive factor;GSF;Insulin promoter factor 1;IPF-1;Insulin upstream factor 1;IUF-1;Islet/duodenum homeobox-1;IDX-1;Somatostatin-transactivating factor 1;STF-1 |
| Human Swissprot No. | 3651 |
| Mouse Gene ID | P52945 |
| Mouse Swissprot No. | 18609 |
| Rat Gene ID | 29535 |
| Rat Swissprot No. | P52947 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 31kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 42kD |
| 同种型 | Rabbit |
| 背景介绍 | The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq, Jul 2008], |
| 细胞定位 | Nucleus. Cytoplasm, cytosol . |
| 功能 | Disease:Defects in PDX1 are a cause of pancreatic agenesis [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.,Disease:Defects in PDX1 are the cause of maturity onset diabetes noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type II.,Disease:Defects in PDX1 are the cause of maturity onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY [MIM:606391] is a form of diabetes mellitus characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion.,Domain:The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.,Domain:The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).,Function:Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.,miscellaneous:According to PubMed:16141209, it may be methylated by SETD7 in vitro. However, the relevance of methylation is unsure in vivo.,PTM:Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.,similarity:Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,subunit:Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP (By similarity). Interacts with the methyltransferase SETD7.,tissue specificity:Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells)., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Nucleus. Cytoplasm, cytosol . |
| 组织表达 | Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). |
| 研究领域 | >>Insulin secretion;>>Type II diabetes mellitus;>>Maturity onset diabetes of the young |
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