| 别名 | Serine/threonine-protein kinase/endoribonuclease IRE1 |
| 基因名称 | IRE1α (Phospho Ser724) Antibody detects endogenous levels of IRE1 protein only when phosphorylated at S724.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RHsFS |
| 蛋白名称 | ERN1 IRE1 |
| 推荐应用 | |
| 反应物种 | IRE1α |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | ERN1;IRE1;Serine/threonine-protein kinase/endoribonuclease IRE1;Endoplasmic reticulum-to-nucleus signaling 1;Inositol-requiring protein 1;hIRE1p;Ire1-alpha;IRE1a |
| Human Swissprot No. | 2081 |
| Mouse Gene ID | O75460 |
| Mouse Swissprot No. | 78943 |
| 特异性 | Monoclonal |
| 稀释度 | Phospho |
| 参考分子量 | 110kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 110kD |
| 同种型 | Rabbit |
| 背景介绍 | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008], |
| 细胞定位 | Endoplasmic reticulum membrane |
| 功能 | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,Function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1.,tissue specificity:Ubiquitously expressed. High levels observed in pancreatic tissue., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Endoplasmic reticulum membrane |
| 组织表达 | Ubiquitously expressed. High levels observed in pancreatic tissue. |
| 研究领域 | >>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Lipid and atherosclerosis |
周一至周日 8:00-18:00
