| 别名 | Histone RNA hairpin-binding protein (Histone stem-loop-binding protein) |
| 基因名称 | Endogenous |
| 蛋白名称 | SLBP,HBP |
| 推荐应用 | |
| 反应物种 | SLBP |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000; |
| Human Gene Link | HBP |
| Human Swissprot No. | 7884 |
| Mouse Gene ID | Q14493 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 30kDa |
| 预测分子量 | WB,IHC,IF,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 30kDa |
| 同种型 | Rabbit |
| 背景介绍 | This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq, Jul 2008] |
| 细胞定位 | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:15829567}. Nucleus {ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:15829567}. Note=Polyribosome-associated (PubMed:12588979). Localizes predominantly in the nucleus at the G1/G2 phases and the beginning of S phase (PubMed:12588979). Through the S phase, partially redistributes to the cytoplasm (PubMed:12588979). Binding to histone mRNA is necessary for cytoplasmic localization (PubMed:12588979). Shuttles between the nucleus and the cytoplasm (PubMed:15829567). Imported in the nucleus by the Importin alpha/Importin beta receptor (PubMed:15829567). {ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:15829567}. |
| 功能 | FUNCTION: RNA-binding protein involved in the histone pre-mRNA processing (PubMed:12588979, PubMed:19155325, PubMed:8957003, PubMed:9049306). Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE) (PubMed:12588979, PubMed:19155325, PubMed:8957003, PubMed:9049306). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery (PubMed:12588979, PubMed:19155325, PubMed:8957003, PubMed:9049306). Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression (PubMed:12588979, PubMed:19155325, PubMed:8957003, PubMed:9049306). Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis (By similarity). Binds to the 5' side of the stem-loop structure of histone pre-mRNAs (By similarity). {ECO:0000250|UniProtKB:P97440, ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:19155325, ECO:0000269|PubMed:8957003, ECO:0000269|PubMed:9049306}. |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:15829567}. Nucleus {ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:15829567}. Note=Polyribosome-associated (PubMed:12588979). Localizes predominantly in the nucleus at the G1/G2 phases and the beginning of S phase (PubMed:12588979). Through the S phase, partially redistributes to the cytoplasm (PubMed:12588979). Binding to histone mRNA is necessary for cytoplasmic localization (PubMed:12588979). Shuttles between the nucleus and the cytoplasm (PubMed:15829567). Imported in the nucleus by the Importin alpha/Importin beta receptor (PubMed:15829567). {ECO:0000269|PubMed:12588979, ECO:0000269|PubMed:15829567}. |
| 组织表达 | TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:9049306}. |
周一至周日 8:00-18:00
