| 别名 | Alpha-actinin-1 |
| 基因名称 | Endogenous |
| 蛋白名称 | ACTN1 |
| 推荐应用 | |
| 反应物种 | Actinin-α1 |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | ACTN1;Alpha-actinin-1;Alpha-actinin cytoskeletal isoform;F-actin cross-linking protein;Non-muscle alpha-actinin-1 |
| Human Swissprot No. | 87 |
| Mouse Gene ID | P12814 |
| Mouse Swissprot No. | 109711 |
| Rat Gene ID | 81634 |
| Rat Swissprot No. | Q9Z1P2 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 103kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 103kD |
| 同种型 | Rabbit |
| 背景介绍 | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| 细胞定位 | Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. . |
| 功能 | Function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cytoplasm, cytoskeleton . Cytoplasm, myofibril, sarcomere, Z line . Cell membrane . Cell junction . Cell projection, ruffle . Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures. . |
| 组织表达 | Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue, |
| 研究领域 | >>Focal adhesion;>>Adherens junction;>>Tight junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Shigellosis;>>Amoebiasis;>>Viral carcinogenesis;>>Systemic lupus erythematosus |
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