| 别名 | Serine/arginine-rich splicing factor 2 (Protein PR264) (Splicing component, 35 kDa) (Splicing factor SC35) (SC-35) (Splicing factor, arginine/serine-rich 2) |
| 基因名称 | Endogenous |
| 蛋白名称 | SRSF2 SFRS2 |
| 推荐应用 | |
| 反应物种 | SRSF2 |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Swissprot No. | 6427 |
| Mouse Gene ID | Q01130 |
| Mouse Swissprot No. | 20382 |
| Rat Gene ID | 20382 |
| Rat Swissprot No. | Q6PDU1 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 25kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 35kD |
| 同种型 | Rabbit |
| 背景介绍 | The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010] |
| 细胞定位 | Nucleus . Nucleus, nucleoplasm . Nucleus speckle . Phosphorylation by SRPK2 provokes its redistribution from the nuclear speckle to nucleoplasm. |
| 功能 | Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Nucleus . Nucleus, nucleoplasm . Nucleus speckle . Phosphorylation by SRPK2 provokes its redistribution from the nuclear speckle to nucleoplasm. |
| 组织表达 | Accumulates in a hypoacetylated/phosphorylated form in response to cisplatin treatment. |
| 研究领域 | >>Spliceosome;>>Herpes simplex virus 1 infection |
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