| 别名 | Serine/threonine-protein kinase PAK 2 |
| 基因名称 | Endogenous |
| 蛋白名称 | PAK2 |
| 推荐应用 | |
| 反应物种 | PAK2 |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000; |
| Human Gene Link | PAK2;Serine/threonine-protein kinase PAK 2;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58 |
| Human Swissprot No. | 5062 |
| Mouse Gene ID | Q13177 |
| Mouse Swissprot No. | 224105 |
| Rat Gene ID | 29432 |
| Rat Swissprot No. | Q64303 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 58kD |
| 预测分子量 | WB,IHC,IF,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 58kD |
| 同种型 | Rabbit |
| 背景介绍 | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008], |
| 细胞定位 | [Serine/threonine-protein kinase PAK 2]: Cytoplasm. MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. |
| 功能 | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylted PAK-2p34 is constitutively active.,Function:The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.,PTM:During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.,PTM:Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.,PTM:PAK-2p34 is myristoylated.,PTM:Ubiquitinated, leading to its proteosomal degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.,subunit:Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with SH3MD4. Interacts with and activated by HIV-1 Nef. PAK-2p34 interacts with ARHGAP10.,tissue specificity:Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG, Kappa |
| 细胞定位 | [Serine/threonine-protein kinase PAK 2]: Cytoplasm. MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. |
| 组织表达 | Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen. |
| 研究领域 | >>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Axon guidance;>>Focal adhesion;>>T cell receptor signaling pathway;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Human immunodeficiency virus 1 infection;>>Renal cell carcinoma |
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