| 别名 | Nuclease-sensitive element-binding protein 1 |
| 基因名称 | Endogenous |
| 蛋白名称 | YBX1 |
| 推荐应用 | |
| 反应物种 | YB-1 |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | YBX1;NSEP1;YB1;Nuclease-sensitive element-binding protein 1;CCAAT-binding transcription factor I subunit A;CBF-A;DNA-binding protein B;DBPB;Enhancer factor I subunit A;EFI-A;Y-box transcription factor;Y-box-binding protein 1;YB- |
| Human Swissprot No. | 4904 |
| Mouse Gene ID | P67809 |
| Mouse Swissprot No. | 22608 |
| Rat Gene ID | 500538 |
| Rat Swissprot No. | P62961 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 36kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 49kD |
| 同种型 | Rabbit |
| 背景介绍 | This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015], |
| 细胞定位 | Cytoplasm . Nucleus . Cytoplasmic granule . Secreted . Secreted, extracellular exosome . Predominantly cytoplasmic in proliferating cells (PubMed:12604611). Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427). Shuttles between nucleus and cytoplasm (PubMed:25229427). Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541). Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673). . |
| 功能 | Function:Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair.,PTM:Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.,PTM:In the absence of phosphorylation the protein is retained in the cytoplasm.,similarity:Contains 1 CSD (cold-shock) domain.,subcellular location:Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus.,subunit:Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG, Kappa |
| 细胞定位 | Cytoplasm . Nucleus . Cytoplasmic granule . Secreted . Secreted, extracellular exosome . Predominantly cytoplasmic in proliferating cells (PubMed:12604611). Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427). Shuttles between nucleus and cytoplasm (PubMed:25229427). Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541). Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673). . |
| 组织表达 | Adrenal cortex,Bone marrow,Brain,Epithelium,Eye,Kidney,Lung,Muscle,Placenta,Skin,Te |
周一至周日 8:00-18:00
