| 别名 | Insulin |
| 基因名称 | Endogenous |
| 蛋白名称 | INS |
| 推荐应用 | |
| 反应物种 | Insulin |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000; |
| Human Swissprot No. | 3630 |
| Mouse Gene ID | P01308 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 12kD |
| 预测分子量 | WB,IHC,IF,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 12kD |
| 同种型 | Rabbit |
| 背景介绍 | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010], |
| 细胞定位 | Cytoplasmic |
| 功能 | Disease:Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].,Function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.,Function:Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.,Function:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.,mass spectrometry: PubMed:12586351; PubMed:15359740,online information:Clinical information on Eli Lilly insulin products,online information:Insulin entry,online information:Insulin-like growth factor 2 entry,online information:Protein of the 20th century - Issue 9 of April 2001,pharmaceutical:Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53.,similarity:Belongs to the insulin family.,subunit:Heterodimer of a B chain and an A chain linked by two disulfide bonds., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cytoplasmic |
| 组织表达 | Blood,Liver,Muscle,Pancreas, |
| 研究领域 | >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Insulin secretion;>>Ovarian steroidogenesis;>>Progesterone-mediated oocyte maturation;>>Prolactin signaling pathway;>>Regulation of lipolysis in adipocytes;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Type I diabetes mellitus;>>Maturity onset diabetes of the young;>>Aldosterone-regulated sodium reabsorption;>>Alzheimer disease;>>Prostate cancer;>>Diabetic cardiomyopathy |
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