| 别名 | PLK1 |
| 基因名称 | Endogenous |
| 蛋白名称 | PLK1 PLK |
| 推荐应用 | |
| 反应物种 | PLK1 |
| 存储缓冲液 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | Serine/threonine-protein kinase PLK1;Polo-like kinase 1;PLK-1;Serine/threonine-protein kinase 13;STPK13; |
| Human Swissprot No. | 5347 |
| Mouse Gene ID | P53350 |
| Mouse Swissprot No. | 18817 |
| Rat Gene ID | 25515 |
| Rat Swissprot No. | Q62673 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 68kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 68kD |
| 同种型 | Rabbit |
| 背景介绍 | The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015], |
| 细胞定位 | Nucleus |
| 功能 | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,developmental stage:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.,enzyme regulation:Activated by serine and threonine phosphorylation.,Function:Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.,induction:By growth-stimulating agents.,PTM:Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.,PTM:Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 POLO box domains.,subunit:Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A.,tissue specificity:Placenta and colon., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Nucleus |
| 组织表达 | Placenta and colon. |
| 研究领域 | >>FoxO signaling pathway;>>Cell cycle;>>Oocyte meiosis;>>Progesterone-mediated oocyte maturation |
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