| 别名 | Glutamate--cysteine ligase catalytic subunit |
| 基因名称 | Endogenous |
| 蛋白名称 | GCLC |
| 推荐应用 | |
| 反应物种 | GCLC |
| 存储缓冲液 | WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| Human Gene Link | GCLC;GLCL;GLCLC;Glutamate--cysteine ligase catalytic subunit;GCS heavy chain;Gamma-ECS;Gamma-glutamylcysteine synthetase |
| Human Swissprot No. | 2729 |
| Mouse Gene ID | P48506 |
| Mouse Swissprot No. | 14629 |
| Rat Gene ID | 25283 |
| Rat Swissprot No. | P19468 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 73kD |
| 预测分子量 | WB,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 73kD |
| 同种型 | Rabbit |
| 背景介绍 | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010], |
| 细胞定位 | Cytoplasm |
| 功能 | Catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,Disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cytoplasm |
| 组织表达 | Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus, |
| 研究领域 | >>Cysteine and methionine metabolism;>>Glutathione metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Ferroptosis |
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