| 别名 | Histone H2B |
| 基因名称 | Endogenous |
| 蛋白名称 | HIST1H2BC H2BFL; HIST1H2BE H2BFH; HIST1H2BF H2BFG; HIST1H2BG H2BFA; HIST1H2BI H2BFK |
| 推荐应用 | |
| 反应物种 | Histone H2B |
| 存储缓冲液 | IHC 1:20000-1:50000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;CHIP 1:50-1:100;Cut&Tag 1:50-1:100; |
| Human Gene Link | Histone H2B type 1-C/E/F/G/I;Histone H2B.1 A;Histone H2B.a;H2B/a;Histone H2B.g;H2B/g;Histone H2B.h;H2B/h;Histone H2B.k;H2B/k;Histone H2B.l;H2B/l; |
| Human Swissprot No. | 3017 |
| Mouse Gene ID | P62807;P58876;Q93079;O60814;Q99880;Q99879;Q99877;Q5QNW6;P57053 |
| Mouse Swissprot No. | 319179 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 14kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA,CHIP,Cut&Tag |
| 运输及保存条件 | Protein A |
| 宿主 | 14kD |
| 同种型 | Rabbit |
| 背景介绍 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| 细胞定位 | Nucleus |
| 功能 | Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,miscellaneous:The mouse orthologous protein seems not to exist.,PTM:Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.,PTM:Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.,similarity:Belongs to the histone H2B family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Nucleus |
| 研究领域 | >>Neutrophil extracellular trap formation;>>Alcoholism;>>Viral carcinogenesis;>>Systemic lupus erythematosus |
周一至周日 8:00-18:00
