| 别名 | Proliferating cell nuclear antigen |
| 基因名称 | Endogenous |
| 蛋白名称 | PCNA |
| 推荐应用 | |
| 反应物种 | PCNA |
| 存储缓冲液 | IHC 1:1000-1:4000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200, |
| Human Gene Link | ATLD2;cb16;Cyclin;DNA polymerase delta auxiliary protein;etID36690.10;fa28e03;fb36g03;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating cell nuclear antigen;wu:fa28e03;wu:fb36g03 |
| Human Swissprot No. | 5111 |
| Mouse Gene ID | P12004 |
| Mouse Swissprot No. | 18538 |
| Rat Gene ID | 25737 |
| Rat Swissprot No. | P04961 |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 29kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 36kD |
| 同种型 | Rabbit |
| 背景介绍 | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008], |
| 细胞定位 | Nucleus |
| 功能 | Disease:Antibodies are present in sera from patients with systemic lupus erythematosus.,Function:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.,online information:PCNA entry,PTM:Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.,similarity:Belongs to the PCNA family.,subunit:Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH. Forms a ternary complex with DNTTIP2 and core histone. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B/WSTF; the interaction is direct., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Nucleus |
| 组织表达 | Tonsil/ Colon |
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