| 别名 | Actin pan |
| 基因名称 | This antibody detects endogenous levels of all actins protein. |
| 蛋白名称 | ACTC1;ACTA1;ACTA2;ACTB;ACTG1;ACTG2 |
| 推荐应用 | |
| 反应物种 | Actin pan |
| 存储缓冲液 | IHC 1:200-1000;WB 1:5000-20000;IF 1:200-1000;ELISA 1:5000-20000;IP 1:50-200 |
| Human Gene Link | Actin, alpha cardiac muscle 1;Alpha-cardiac actin;ACTC;Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;Actin, aortic smooth muscle;Alpha-actin-2;ACTA2;ACTSA, ACTVS;Actin, cytoplasmic 1;Beta-actin;ACTB;Actin, cytoplasmic 2;Gamma-actin;ACTG1;ACTG;Actin, gamma-enteric smooth muscle;Alpha-actin-3;Gamma-2-actin;Smooth muscle gamma-actin;ACTA3, ACTL3, ACTSG;ACTG2 |
| Human Swissprot No. | 70;58;59;60;71;72; |
| Mouse Gene ID | P62736;P68032;P60709;P63261;P68133;P63267 |
| Mouse Swissprot No. | 11475;11464;11465;11468;11459;11461; |
| 特异性 | Monoclonal |
| 稀释度 | Unmodified |
| 参考分子量 | 42kD |
| 预测分子量 | WB,IHC,IF,IP,ELISA |
| 运输及保存条件 | Protein A |
| 宿主 | 42kD |
| 同种型 | Rabbit |
| 背景介绍 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility . While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids. For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. Research studies have shown that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies. Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis. |
| 细胞定位 | Cytoplasmic, Membranous |
| 功能 | Disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,Function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the actin family.,subunit:Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Interacts with XPO6., |
| 纯化 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Clonality | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 修饰类型 | IgG,Kappa |
| 细胞定位 | Cytoplasmic, Membranous |
| 组织表达 | B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car |
| 研究领域 | >>Vascular smooth muscle contraction;>>Apelin signaling pathway;>>Relaxin signaling pathway |
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