| 别名 | Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1 |
| 基因名称 | RB1 |
| 蛋白名称 | Retinoblastoma-associated protein |
| 推荐应用 | |
| 反应物种 | Human（WB);Human Mouse（IHC) |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| 特异性 | Endogenous |
| 稀释度 | WB 1:500-1:2000;IHC-P 1:100-1:500 |
| 参考分子量 | MW(Observed):106kD |
| 预测分子量 | MW(Calculated):106kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). |
| 细胞定位 | Nucleus |
| 功能 | Disease:Defects in RB1 are a cause of bladder cancer [MIM:109800]. ,Disease:Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500]. ,Disease:Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically , about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated. ,Function:Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated , active form of RB1 interacts with E2F1 and represses its transcription activity , leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and , in particular , that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1 , SUV420H1 and SUV420H2 , leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections , interactions with SV40 large T antigen , HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. ,online information:RB1 mutation db ,online information:Retinoblastoma protein entry ,PTM:Phosphorylated in G1 , thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen , HPV E7 and adenovirus E1A bind to the underphosphorylated , active form of pRb. ,similarity:Belongs to the retinoblastoma protein (RB) family. ,subunit:Interacts with ATAD5 (By similarity) . The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. The unphosphorylated form interacts with ARID3B , KDM5A , SUV39H1 , MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF , DNMT1 , LIN9 , LMNA , SUV420H1 , SUV420H2 , PELP1 and TMPO-alpha. May interact with NDC80. Interacts with EID1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1. Interacts with adenovirus E1A protein , HPV E7 protein and SV40 large T antigen. |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Nucleus |
| 组织表达 | Testis, Bone marrow, Intestinal epithelium, Retinoblastoma, Lung cancer, Osteosarcoma |
周一至周日 8:00-18:00
