| 别名 | CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1 |
| 基因名称 | MMP1 |
| 蛋白名称 | Interstitial collagenase |
| 推荐应用 | |
| 反应物种 | Human |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Swissprot No. | P03956 |
| 特异性 | Endogenous |
| 稀释度 | IHC 1:100-1:300 |
| 预测分子量 | 54kDa |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | matrix metallopeptidase 1 (MMP1) Homo sapiens This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs) . Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes , such as embryonic development , reproduction , and tissue remodeling , as well as in disease processes , such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens , including types I , II , and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD) . Alternative splicing results in multiple transcript variants , at least one of which encodes an isoform that is proteolytically processed. |
| 细胞定位 | Cytoplasm, Secreted |
| 功能 | Catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus , at 775-Gly-|-Ile-776 in the alpha-1 (I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue. ,cofactor:Binds 2 zinc ions per subunit. ,cofactor:Binds 4 calcium ions per subunit. ,Domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion , thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. ,Domain:There are two distinct domains in this protein; the catalytic N-terminal , and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases) . ,enzyme regulation:Can be activated without removal of the activation peptide. ,Function:Cleaves collagens of types I , II , and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection , interacts and cleaves the secreted viral Tat protein , leading to a decrease in neuronal Tat's mediated neurotoxicity. |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cytoplasm, Secreted |
| 组织表达 | Fibroblasts, Endothelial cells, Macrophages, Breast carcinoma, Lung cancer, Colorectal carcinoma, Melanoma |
周一至周日 8:00-18:00
