| 别名 | IFIH1;MDA5;RH116;Interferon-induced helicase C domain-containing protein 1;Clinically amyopathic dermatomyositis autoantigen 140 kDa;CADM-140 autoantigen;Helicase with 2 CARD domains;Helicard;Interferon-induced with helicase C domai |
| 基因名称 | MDA5 |
| 蛋白名称 | Interferon-induced helicase C domain-containing protein 1 |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 64135 |
| Human Swissprot No. | Q9BYX4 |
| Mouse Gene ID | 71586 |
| Mouse Swissprot No. | Q8R5F7 |
| 特异性 | endogenous |
| 稀释度 | WB 1:1000-1:5000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):135kD |
| 预测分子量 | MW(Calculated):117kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-depend |
| 细胞定位 | Cytoplasm . Nucleus . Mitochondrion . Upon viral RNA stimulation and ISGylation, translocates from cytosol to mitochondrion. May be found in the nucleus, during apoptosis. |
| 功能 | Disease:Genetic variation in IFIH1 is associated with insulin-dependent diabetes mellitus 19 (IDDM19) [MIM:610155].,Function:RNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.,induction:By IFN-beta and TNF-alpha.,miscellaneous:In HIV-1 infected HeLa-CD4 cells, overexpression of IFIH1 results in a great increase in the level of secreted viral p24 protein.,PTM:During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 CARD domains.,subcellular location:May be found in the nucleus, during apoptosis.,subunit:Interacts with MAVS. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state.,tissue specificity:Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cytoplasm . Nucleus . Mitochondrion . Upon viral RNA stimulation and ISGylation, translocates from cytosol to mitochondrion. May be found in the nucleus, during apoptosis. |
| 组织表达 | Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. |
| 研究领域 | >>RIG-I-like receptor signaling pathway;>>Hepatitis B;>>Measles;>>Influenza A;>>Herpes simplex virus 1 infection;>>Coronavirus disease - COVID-19 |
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