| 别名 | SPAM1;HYAL3;PH20;Hyaluronidase PH-20 ;Hyal-PH20;Hyaluronoglucosaminidase PH-20;Sperm adhesion molecule 1;Sperm surface protein PH-20; |
| 基因名称 | SPAM1 |
| 蛋白名称 | Hyaluronidase PH-20;Hyal-PH20;Hyaluronoglucosaminidase PH-20;Sperm adhesion molecule 1;Sperm surface protein PH-20; |
| 推荐应用 | |
| 反应物种 | Human |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 6677 |
| Human Swissprot No. | P38567 |
| Mouse Gene ID | 20690 |
| Mouse Swissprot No. | P48794 |
| Rat Gene ID | 117037 |
| Rat Swissprot No. | Q62803 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:100-1:500;WB 1:1000-1:2500； |
| 参考分子量 | MW(Observed):56kD |
| 预测分子量 | MW(Calculated):58kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Hyaluronidase degrades hyaluronic acid , a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte , a receptor that plays a role in hyaluronic acid induced cell signaling , and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 功能 | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. {ECO:0000269|PubMed:8282124}. |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 组织表达 | Testis. {ECO:0000269|PubMed:8234258}. |
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