| 别名 | PDPK1;PDK1;3-phosphoinositide-dependent protein kinase 1;hPDK1 |
| 基因名称 | PDPK1 |
| 蛋白名称 | 3-phosphoinositide-dependent protein kinase 1 |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 5170 |
| Human Swissprot No. | O15530 |
| Mouse Gene ID | 18607 |
| Mouse Swissprot No. | Q9Z2A0 |
| Rat Gene ID | 81745 |
| Rat Swissprot No. | O55173 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:200-1:1000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):58-68kD |
| 预测分子量 | MW(Calculated):63kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously., |
| 细胞定位 | Membrane, Cytoplasm |
| 功能 | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Membrane, Cytoplasm |
| 组织表达 | Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. |
| 研究领域 | >>Platinum drug resistance;>>PPAR signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Apoptosis;>>Axon guidance;>>Focal adhesion;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Neurotrophin signaling pathway;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Insulin resistance;>>Aldosterone-regulated sodium reabsorption;>>Toxoplasmosis;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Endometrial cancer;>>Prostate cancer;>>Non-small cell lung cancer;>>Choline metabolism in cancer;>>Lipid and atherosclerosis |
周一至周日 8:00-18:00
