| 别名 | TOP1;DNA topoisomerase 1;DNA topoisomerase I |
| 基因名称 | Topoisomerase I |
| 蛋白名称 | DNA topoisomerase 1 |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 7150 |
| Human Swissprot No. | P11387 |
| Mouse Gene ID | 21969 |
| Mouse Swissprot No. | Q04750 |
| Rat Gene ID | 64550 |
| Rat Swissprot No. | Q9WUL0 |
| 特异性 | endogenous |
| 稀释度 | WB 1:1000-1:4000;IF 1:50-1:200;ELISA 1:5000-1:20000; |
| 参考分子量 | MW(Observed):91kD |
| 预测分子量 | MW(Calculated):91kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008], |
| 细胞定位 | Nucleus, nucleolus . Nucleus, nucleoplasm . Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli. |
| 功能 | Catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,Disease:A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.,enzyme regulation:Specifically inhibited by camptothecin (CPT), a plant alkaloid with antitumor activity.,Function:The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,miscellaneous:When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.,PTM:Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.,similarity:Belongs to the eukaryotic type I topoisomerase family.,subcellular location:Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.,subunit:Monomer. Interacts with SV40 Large T antigen; this interactions allows viral DNA replication., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Nucleus, nucleolus . Nucleus, nucleoplasm . Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli. |
| 组织表达 | Endothelial cells. |
周一至周日 8:00-18:00
