| 别名 | Folylpoly-gamma-glutamate synthetase, Tetrahydrofolylpolyglutamate synthase, FPGS, Tetrahydrofolate synthase |
| 基因名称 | FPGS |
| 蛋白名称 | Folylpolyglutamate synthase, mitochondrial (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) (Tetrahydrofolate synthase) |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 2356 |
| Human Swissprot No. | Q05932 |
| Mouse Gene ID | 14287 |
| Mouse Swissprot No. | P48760 |
| 特异性 | endogenous |
| 稀释度 | WB 1:1000-1:2500;FC 1:100-1:500; |
| 参考分子量 | MW(Observed):65kDa |
| 预测分子量 | MW(Calculated):65kDa |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014] |
| 细胞定位 | SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion inner membrane {ECO:0000269|PubMed:16169100}. Mitochondrion matrix {ECO:0000269|PubMed:16169100, ECO:0000269|PubMed:8408020, ECO:0000269|PubMed:8662720}.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm {ECO:0000269|PubMed:8662720}. |
| 功能 | FUNCTION: Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates. {ECO:0000269|PubMed:8408018, ECO:0000269|PubMed:8408019, ECO:0000269|PubMed:8408021, ECO:0000269|PubMed:8662720}. |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion inner membrane {ECO:0000269|PubMed:16169100}. Mitochondrion matrix {ECO:0000269|PubMed:16169100, ECO:0000269|PubMed:8408020, ECO:0000269|PubMed:8662720}.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm {ECO:0000269|PubMed:8662720}. |
| 组织表达 | Liver, Kidney, Bone marrow, Leukemia, Colon cancer, Breast cancer |
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