| 别名 | ZNF364 |
| 基因名称 | ZNF364 |
| 蛋白名称 | E3 ubiquitin-protein ligase RNF115 (EC 2.3.2.27) (RING finger protein 115) (RING-type E3 ubiquitin transferase RNF115) (Rab7-interacting RING finger protein) (Rabring 7) (Zinc finger protein 364) |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 27246 |
| Human Swissprot No. | Q9Y4L5 |
| Mouse Gene ID | 67845 |
| Mouse Swissprot No. | Q9D0C1 |
| 特异性 | endogenous |
| 稀释度 | WB 1:1000-1:2500; |
| 参考分子量 | MW(Observed):33kDa |
| 预测分子量 | MW(Calculated):33kDa |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Enables ubiquitin protein ligase activity. Involved in negative regulation of signal transduction; protein ubiquitination; and ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Is active in endoplasmic reticulum. [provided by Alliance of Genome Resources, Apr 2025] |
| 细胞定位 | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021}. Nucleus {ECO:0000269|PubMed:24852021}. Endoplasmic reticulum {ECO:0000269|PubMed:35343654}. Golgi apparatus {ECO:0000269|PubMed:35343654}. Note=The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes. {ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021}. |
| 功能 | FUNCTION: E3 ubiquitin-protein ligase that catalyzes the 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of various substrates and thereby plays a role in a number of signaling pathways including autophagy, innate immunity, cell proliferation and cell death (PubMed:20019814, PubMed:30689267). Plays a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4 through their polyubiquitination. Participates together with BST2 in antiviral immunity by facilitating the internalization of HIV-1 virions into intracellular vesicles leading to their lysosomal degradation (PubMed:20019814). Possesses also an antiviral activity independently of BST2 by promoting retroviral GAG proteins ubiquitination, redistribution to endo-lysosomal compartments and, ultimately, lysosomal degradation (PubMed:24852021). Catalyzes distinct types of ubiquitination on MAVS and STING1 at different phases of viral infection to promote innate antiviral response (PubMed:33139700). Mediates the 'Lys-48'-linked ubiquitination of MAVS leading to its proteasomal degradation and ubiquitinates STING1 via 'Lys-63'-linked polyubiquitination, critical for its oligomerization and the subsequent recruitment of TBK1 (PubMed:33139700). Plays a positive role in the autophagosome-lysosome fusion by interacting with STX17 and enhancing its stability without affecting 'Lys-48'- or 'Lys-63'-linked polyubiquitination levels, which in turn promotes autophagosome maturation (PubMed:32980859). Negatively regulates TLR-induced expression of proinflammatory cytokines by catalyzing 'Lys-11'-linked ubiquitination of RAB1A and RAB13 to inhibit post-ER trafficking of TLRs to the Golgi by RAB1A and subsequently from the Golgi apparatus to the cell surface by RAB13 (PubMed:35343654). {ECO:0000269|PubMed:16288031, ECO:0000269|PubMed:18819927, ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021, ECO:0000269|PubMed:32980859, ECO:0000269|PubMed:33139700, ECO:0000269|PubMed:35343654, ECO:0000303|PubMed:23418353}. |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021}. Nucleus {ECO:0000269|PubMed:24852021}. Endoplasmic reticulum {ECO:0000269|PubMed:35343654}. Golgi apparatus {ECO:0000269|PubMed:35343654}. Note=The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes. {ECO:0000269|PubMed:20019814, ECO:0000269|PubMed:24852021}. |
| 组织表达 | TISSUE SPECIFICITY: Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells. {ECO:0000269|PubMed:16288031}. |
周一至周日 8:00-18:00
