| 别名 | DNA (cytosine-5)-methyltransferase 3A、DNMT3A、DNA methyltransferase HsaIIIA、DNA MTase HsaIIIA、M.HsaIIIA、DNMT3A2、MCMT、TBRS |
| 基因名称 | DNM3A |
| 蛋白名称 | DNA (cytosine-5)-methyltransferase 3A (Dnmt3a) (DNA methyltransferase HsaIIIA) (DNA MTase HsaIIIA) (M.HsaIIIA) |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 1788 |
| Human Swissprot No. | Q9Y6K1 |
| Mouse Swissprot No. | O88508 |
| Rat Swissprot No. | Q1LZ53 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:100-1:500;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):130kD |
| 预测分子量 | MW(Calculated):102kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016], |
| 细胞定位 | Nucleus . Chromosome . Cytoplasm . Accumulates in the major satellite repeats at pericentric heterochromatin. . |
| 功能 | Catalytic activity:S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.,Caution:It is uncertain whether Met-1 or Met-4 is the initiator.,Function:Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones.,similarity:Belongs to the C5-methyltransferase family.,similarity:Contains 1 ADD-type zinc finger.,similarity:Contains 1 PWWP domain.,subunit:Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD-type zinc-finger (By similarity). Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex.,tissue specificity:Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Nucleus . Chromosome . Cytoplasm . Accumulates in the major satellite repeats at pericentric heterochromatin. . |
| 组织表达 | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
| 研究领域 | >>Cysteine and methionine metabolism;>>Metabolic pathways;>>MicroRNAs in cancer |
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