| 别名 | Neutrophil elastase、ELANE、ELA2、Leukocyte elastase、Bone marrow serine protease、PMN-E、PMN elastase |
| 基因名称 | ELNE |
| 蛋白名称 | Neutrophil elastase (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| 推荐应用 | |
| 反应物种 | Human |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 1991 |
| Human Swissprot No. | P08246 |
| Mouse Swissprot No. | Q3UP87 |
| 特异性 | endogenous |
| 稀释度 | WB 1:1000-1:5000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):30kD |
| 预测分子量 | MW(Calculated):29kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016 |
| 细胞定位 | Cytoplasmic vesicle, phagosome . Localized in phagolysosomes following ingestion of E.coli by neutrophils. . |
| 功能 | Catalytic activity:Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.,Disease:Defects in ELA2 are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.,Disease:Defects in ELA2 are the cause of autosomal dominant severe congenital neutropenia type 1 (SCN1) [MIM:202700]. Severe congenital neutropenia is a heterogeneous disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.,Function:Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.,online information:ELA2 mutation db,online information:Elastase entry,similarity:Belongs to the peptidase S1 family. Elastase subfamily.,similarity:Contains 1 peptidase S1 domain.,subunit:Interacts with NOTCH2NL.,tissue specificity:Bone marrow cells., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cytoplasmic vesicle, phagosome . Localized in phagolysosomes following ingestion of E.coli by neutrophils. . |
| 组织表达 | Bone marrow cells. Neutrophil (PubMed:10947984). |
| 研究领域 | >>Neutrophil extracellular trap formation;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus |
周一至周日 8:00-18:00
