| 别名 | RPA2;REPA2;RPA32;RPA34;Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34 |
| 基因名称 | RPA2 |
| 蛋白名称 | Replication protein A 32 kDa subunit |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat, |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 6118 |
| Human Swissprot No. | P15927 |
| Mouse Swissprot No. | Q62193 |
| Rat Gene ID | 59102 |
| Rat Swissprot No. | Q63528 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:1000-1:4000;WB 1:1000-1:2500;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200, |
| 参考分子量 | MW(Observed):29kD |
| 预测分子量 | MW(Calculated):29kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., |
| 细胞定位 | Nucleus |
| 功能 | Function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.,subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Nucleus |
| 组织表达 | Kidney,Lung,Muscle, |
| 研究领域 | >>DNA replication;>>Nucleotide excision repair;>>Mismatch repair;>>Homologous recombination;>>Fanconi anemia pathway |
周一至周日 8:00-18:00
