| 别名 | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial;PHGPx;Glutathione peroxidase 4;GPx-4;GSHPx-4; |
| 基因名称 | GPX4 |
| 蛋白名称 | GPX4 |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat, |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 2879 |
| Human Swissprot No. | P36969 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:200-1:1000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):22kD |
| 预测分子量 | MW(Calculated):22kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016], |
| 细胞定位 | Cytoplasm |
| 功能 | Catalytic activity:2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H(2)O.,Disease:Defects in GPX4 may be a cause of infertility.,Function:Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.,similarity:Belongs to the glutathione peroxidase family.,subunit:Monomer. Has a tendency to form higher mass oligomers.,tissue specificity:Present primarily in testis., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cytoplasm |
| 组织表达 | Present primarily in testis. Expressed in platelets (at protein level) (PubMed:11115402). |
| 研究领域 | >>Glutathione metabolism;>>Metabolic pathways;>>Ferroptosis |
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