| 别名 | PRKCD;Protein kinase C delta type;Tyrosine-protein kinase PRKCD;nPKC-delta |
| 基因名称 | PRKCD |
| 蛋白名称 | Protein kinase C delta type |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat, |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 5580 |
| Human Swissprot No. | Q05655 |
| Mouse Gene ID | 18753 |
| Mouse Swissprot No. | P28867 |
| Rat Gene ID | 170538 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:500-1:2000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):78kD |
| 预测分子量 | MW(Calculated):78kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008], |
| 细胞定位 | Cytoplasm, Nucleus |
| 功能 | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.,Domain:The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.,enzyme regulation:Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation.,Function:This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.,PTM:Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with PDK1, RAD9A, CDCP1 and MUC1., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cytoplasm, Nucleus |
| 组织表达 | Epithelium,Hippocampus,Liver,Platelet,Skin, |
| 研究领域 | >>Chemokine signaling pathway;>>Autophagy - animal;>>Vascular smooth muscle contraction;>>NOD-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Neurotrophin signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Estrogen signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Prion disease;>>Shigellosis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy |
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