| 别名 | GAPDH;GAPD;CDABP0047;OK/SW-cl.12;Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;Peptidyl-cysteine S-nitrosylase GAPDH |
| 基因名称 | GAPDH |
| 蛋白名称 | Glyceraldehyde-3-phosphate dehydrogenase |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat, |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 2597 |
| Human Swissprot No. | P04406 |
| Mouse Gene ID | 100042025 |
| Mouse Swissprot No. | P16858 |
| Rat Gene ID | 24383 |
| Rat Swissprot No. | P04797 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:2000-1:5000;WB 1:5000-1:25000;IF 1:50-1:200;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):38kD |
| 预测分子量 | MW(Calculated):38kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri |
| 细胞定位 | Cytoplasm, Nucleus |
| 功能 | Catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,Function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence Caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.,subcellular location:Postnuclear and Perinuclear regions.,subunit:Homotetramer.,subunit:Homotetramer. Binds PRKCI., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Cytoplasm, Nucleus |
| 组织表达 | Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu |
| 研究领域 | >>Glycolysis / Gluconeogenesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>HIF-1 signaling pathway;>>Alzheimer disease;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Diabetic cardiomyopathy |
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