| 别名 | E2F1;RBBP3;Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1 |
| 基因名称 | E2F-1 |
| 蛋白名称 | Transcription factor E2F1 |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat, |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | 1869 |
| Human Swissprot No. | Q01094 |
| Mouse Gene ID | 13555 |
| Mouse Swissprot No. | Q61501 |
| 特异性 | endogenous |
| 稀释度 | IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | MW(Observed):60kD |
| 预测分子量 | MW(Calculated):47kD |
| 运输及保存条件 | -15°C to -25°C/1 year(Do not lower than -25°C) |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | The protein encoded by this gene is a member ofThe E2F family of transcription factors.The E2F family plays a crucial role inThe control of cell cycle and action of tumor suppressor proteins and is also a target ofThe transforming proteins of small DNA tumor viruses.The E2F proteins contain several evolutionally conserved domains found in most members ofThe family. These domains include a DNA binding domain, a dimerization domain which determines interaction withThe differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded withinThe transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media |
| 细胞定位 | Nucleus |
| 功能 | Function:Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A., |
| 纯化 | Protein A |
| Clonality | Monoclonal |
| 修饰类型 | Unmodified |
| 细胞定位 | Nucleus |
| 组织表达 | Brain,Epithelium,Pancreas,Skin, |
| 研究领域 | >>Endocrine resistance;>>Cell cycle;>>Mitophagy - animal;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
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