| 别名 | Neuro-oncological ventral antigen 1;Onconeural ventral antigen 1;Paraneoplastic Ri antigen;Ventral neuron-specific protein 1 |
| 基因名称 | NOVA1 |
| 蛋白名称 | RNA-binding protein Nova-1 |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Swissprot No. | P51513 |
| Mouse Swissprot No. | Q9JKN6 |
| Rat Swissprot No. | Q80WA4 |
| 特异性 | Endogenous |
| 稀释度 | IHC-P 1:200-1:1000;WB 1:2000-1:1000;IF-P/IF-F/IF-ICC 1:200-1:1000;ELISA 1:5000-1:20000 |
| 参考分子量 | 58kDa |
| 预测分子量 | 52kDa |
| 运输及保存条件 | Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed : 10811881) . Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA , blocking U1 snRNP binding and exon inclusion , whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA , blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion , whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1 , they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord (By similarity) . |
| 细胞定位 | Nucleus . |
| 纯化 | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Clonality | Monoclonal |
| 细胞定位 | Nucleus . |
周一至周日 8:00-18:00
