| 别名 | PKMYT1;MYT1;Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase;Myt1 kinase |
| 基因名称 | PKMYT1 |
| 蛋白名称 | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Swissprot No. | Q99640 |
| Mouse Swissprot No. | Q9ESG9 |
| 特异性 | Endogenous |
| 稀释度 | IHC-P 1:200-1:500;WB 1:1000-1:5000;IF-P/IF-F/IF-ICC 1:200-1:1000;ELISA 1:5000-1:20000; |
| 参考分子量 | 70kDa |
| 预测分子量 | 55kDa |
| 运输及保存条件 | Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012], |
| 细胞定位 | Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein . |
| 功能 | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,Function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.,PTM:Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.,sequence Caution:Chimeric cDNA.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1., |
| Clonality | Monoclonal |
| 细胞定位 | Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein . |
周一至周日 8:00-18:00
