| 别名 | Shank-associated RH domain-interacting protein;Shank-interacting protein-like 1;hSIPL1;SIPL1;PSEC0216 |
| 基因名称 | SHARPIN |
| 蛋白名称 | Sharpin |
| 推荐应用 | |
| 反应物种 | Human |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Swissprot No. | Q9H0F6 |
| Mouse Gene ID | 106025 |
| Mouse Swissprot No. | Q91WA6 |
| Rat Gene ID | 81859 |
| Rat Swissprot No. | Q9EQL9 |
| 特异性 | Endogenous |
| 稀释度 | WB 1:1000-1:5000;IF-P/IF-F/IF-ICC 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200; |
| 参考分子量 | 43kDa |
| 预测分子量 | 40kDa |
| 运输及保存条件 | Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles |
| 宿主 | Rabbit |
| 同种型 | IgG,Kappa |
| 背景介绍 | Enables polyubiquitin modification-dependent protein binding activity. Involved in defense response to bacterium; protein linear polyubiquitination; and regulation of signal transduction. Located in cytosol. Part of LUBAC complex. [provided by Alliance of Genome Resources, Jul 2025] |
| 细胞定位 | Cytoplasm, cytosol . Cell junction, synapse . Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. . |
| 功能 | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation . LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways . Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation . LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex . The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria . LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin . The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation . Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis . |
| Clonality | Monoclonal |
| 细胞定位 | Cytoplasm, cytosol . Cell junction, synapse . Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. . |
周一至周日 8:00-18:00
