| 别名 | C20orf21;DACA 1;DACA-1;Dermatomyositis associated with cancer putative autoantigen 1;YTH domain family 1;YTH domain family member 1;YTH domain family protein 1;YTHD1;YTHD1_HUMAN;Ythdf1; |
| 基因名称 | Ythdf1 |
| 蛋白名称 | YTH domain-containing family protein 1 (Dermatomyositis associated with cancer putative autoantigen 1 homolog) (DACA-1 homolog) |
| 推荐应用 | |
| 反应物种 | Human,Mouse,Rat |
| 存储缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Human Gene Link | http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54915 |
| Human Swissprot No. | Q9BYJ9 |
| Human Swissprot Link | http://www.uniprot.org/uniprotkb/Q9BYJ9 |
| Rat Swissprot No. | P59326 |
| Rat Swissprot Link | https://www.uniprot.org/uniprotkb/P59326/entry |
| 稀释度 | IHC-P 1:200-1:1000,WB 1:1000-1:5000,IF-P/IF-F/IF-ICC 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200 |
| 参考分子量 | 70kDa |
| 预测分子量 | 61kDa |
| 运输及保存条件 | Biological ice bag transportation. Store at -20°C for at least 12 months(Do not lower than -25°C). Avoid freeze/thaw cycles |
| 宿主 | Rabbit |
| 同种型 | Monoclonal, rabbit, IgG, Kappa |
| 背景介绍 | Enables N6-methyladenosine-containing RNA binding activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule. [provided by Alliance of Genome Resources, Apr 2022] |
| 功能 | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability (PubMed:30401835, PubMed:32943573). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed:30401835, PubMed:32943573). Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex (By similarity). The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation (PubMed:32943573). Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs (PubMed:30401835). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts (PubMed:30843071). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (PubMed:30728504). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (PubMed:30728504). Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation (By similarity). The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules (By similarity). |
| 纯化 | Protein A |
| Clonality | Monoclonal |
周一至周日 8:00-18:00
