货号:GZEL52970
CD172a; SIRP-A; P84; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; Macrophage fusion receptor; Tyrosine-protein phosphatase non-receptor type substrate 1; SHP substrate 1;SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; MyD-1 antigen; Signal regulatory protein alpha type 1; Bit; Brain Ig like molecule with tyrosine based activation motifs; Brain immunoglobulin like molecule with tyrosine based activation motifs; CD172 antigen like family member A; CD172 antigen-like family member A; CD172a antigen; Inhibitory receptor SHPS-1; MYD 1; Myd 1 antigen; p84; Protein tyrosine phosphatase non receptor type substrate 1; SHPS1; SHPS1_HUMAN; Signal regulatory protein alpha; Signal regulatory protein alpha 2; Signal regulatory protein alpha 3; Signal regulatory protein alpha type 2; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SIRPA; SIRPalpha; SIRPalpha1; SIRPalpha2; SIRPalpha3; Tyrosine phosphatase SHP substrate 1; Tyrosine protein phosphatase non receptor type substrate 1
我司质检部门依据产品标准,在受控的实验室环境(环境温度:18-25°C,相对湿度:35-75%)和严格的实验操作(TMB 37°C避光反应1818分钟后终止读数)下完成检测,确保产品性能符合要求。因具体实验环境条件及实验操作存在差异,以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
| 浓度(ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0.156 | 0 |
|---|---|---|---|---|---|---|---|---|
| OD值 | 2.52 | 1.96 | 1.38 | 0.96 | 0.55 | 0.31 | 0.19 | 0.08 |
分别往不同样本中添加已知浓度的信号调节蛋白α(SIRPα),做回收实验,得出回收率范围和平均回收率
| 样本类型 | 范围 | 平均回收率 |
|---|---|---|
| 血清(n=8) | 92.4-105.0 | 102.9 |
| 血浆(n=8) | 96.8-108.9 | 105.6 |
| 细胞培养上清(n=8) |
将添加有信号调节蛋白α(SIRPα)的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
| 稀释比例 | 回收率(%) | 血清 | 血浆 | 细胞培养上清 |
|---|---|---|---|---|
| 1:2 | 范围(%) | 84.5-96.3 | 90.2-110.8 | |
| 平均回收率(%) | 93.8 | 96.2 | ||
| 1:4 | 范围(%) | 87.9-108.1 | 105.9-115.4 | |
| 平均回收率(%) | 100.4 | 108.1 |