货号:GZEL25385
DPC4; JIP; MADH4; Deletion target in pancreatic carcinoma 4; (Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma; Deleted in Pancreatic Carcinoma 4; Deleted in pancreatic carcinoma locus 4; DPC 4; hSMAD4; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic, Drosophila, homolog of, 4; Mothers against DPP homolog 4; MYHRS; OTTHUMP00000163548; SMA- and MAD-related protein 4; SMAD 4; SMAD family member 4; SMAD mothers against DPP homolog 4; SMAD4; SMAD4_HUMAN
我司质检部门依据产品标准,在受控的实验室环境(环境温度:18-25°C,相对湿度:35-75%)和严格的实验操作(TMB 37°C避光反应1658分钟后终止读数)下完成检测,确保产品性能符合要求。因具体实验环境条件及实验操作存在差异,以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
| 浓度(ng/mL) | 20 | 10 | 5 | 2.5 | 1.25 | 0.62 | 0.31 | 0 |
|---|---|---|---|---|---|---|---|---|
| OD值 | 2.56 | 1.71 | 1.19 | 0.79 | 0.46 | 0.3 | 0.23 | 0.08 |
分别往不同样本中添加已知浓度的Smad同源物4(Smad4),做回收实验,得出回收率范围和平均回收率
| 样本类型 | 范围 | 平均回收率 |
|---|---|---|
| 血清(n=8) | 84.2-101.0 | 95.2 |
| 血浆(n=8) | 92.4-105.5 | 102.2 |
| 细胞培养上清(n=8) |
将添加有Smad同源物4(Smad4)的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
| 稀释比例 | 回收率(%) | 血清 | 血浆 | 细胞培养上清 |
|---|---|---|---|---|
| 1:2 | 范围(%) | 84.9-95.5 | 88.8-96.2 | |
| 平均回收率(%) | 91.4 | 93.4 | ||
| 1:4 | 范围(%) | 89.6-103.5 | 87.4-108.4 | |
| 平均回收率(%) | 94.8 | 98.9 |