货号:GZEL47404
MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II; COX 2; COX-2; COX2; Cyclooxygenase; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase-2; Cyclooxygenase2; EC 1.14.99.1; fj02a10; Glucocorticoid-regulated inflammatory cyclooxygenase; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; hCox 2; Macrophage activation-associated marker protein P71/73; OTTHUMP00000033524; PES-2; PGG/HS; PGH synthase 2; PGH2_HUMAN; PGHS 2; PGHS-2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin G/H synthase; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); PTGS2; ptgs2a; TIS10; TIS10 protein; unp1239; wu:fj02a10
我司质检部门依据产品标准,在受控的实验室环境(环境温度:18-25°C,相对湿度:35-75%)和严格的实验操作(TMB 37°C避光反应410分钟后终止读数)下完成检测,确保产品性能符合要求。因具体实验环境条件及实验操作存在差异,以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
| 浓度(ng/mL) | 20 | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0 |
|---|---|---|---|---|---|---|---|---|
| OD值 | 2.31 | 1.52 | 1.01 | 0.63 | 0.44 | 0.32 | 0.24 | 0.09 |
分别往不同样本中添加已知浓度的细胞色素C氧化酶亚基Ⅱ(COX2),做回收实验,得出回收率范围和平均回收率
| 样本类型 | 范围 | 平均回收率 |
|---|---|---|
| 血清(n=8) | 92.9-105.5 | 100.7 |
| 血浆(n=8) | 95.1-108.3 | 105.2 |
| 细胞培养上清(n=8) |
将添加有细胞色素C氧化酶亚基Ⅱ(COX2)的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
| 稀释比例 | 回收率(%) | 血清 | 血浆 | 细胞培养上清 |
|---|---|---|---|---|
| 1:2 | 范围(%) | 88.9-96.1 | 90.7-110.4 | |
| 平均回收率(%) | 90.6 | 97.5 | ||
| 1:4 | 范围(%) | 87.6-106.3 | 105.4-115.7 | |
| 平均回收率(%) | 100.7 | 107.2 |