货号:GZEL21044
PGG/HS; PGHS2; PHS2; HCox2; PTGS2; Prostaglandin Endoperoxide Synthase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; COX 2; COX2; Cyclooxygenase; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase2; EC 1.14.99.1; fj02a10; Glucocorticoid-regulated inflammatory cyclooxygenase; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; hCox 2; Macrophage activation-associated marker protein P71/73; OTTHUMP00000033524; PES-2; PGH synthase 2; PGH2_HUMAN; PGHS 2; PGHS-2; PHS 2; PHS II; Prostaglandin endoperoxide synthase 2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin G/H synthase; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); ptgs2a; TIS10; TIS10 protein; unp1239; wu:fj02a10
我司质检部门依据产品标准,在受控的实验室环境(环境温度:18-25°C,相对湿度:35-75%)和严格的实验操作(TMB 37°C避光反应319分钟后终止读数)下完成检测,确保产品性能符合要求。因具体实验环境条件及实验操作存在差异,以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
| 浓度(ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0.156 | 0 |
|---|---|---|---|---|---|---|---|---|
| OD值 | 2 | 1.56 | 1.14 | 0.76 | 0.49 | 0.33 | 0.24 | 0.1 |
分别往不同样本中添加已知浓度的环氧合酶2(COX-2),做回收实验,得出回收率范围和平均回收率
| 样本类型 | 范围 | 平均回收率 |
|---|---|---|
| 血清(n=8) | 85.7-101.0 | 97.5 |
| 血浆(n=8) | 92.9-105.2 | 102.9 |
| 细胞培养上清(n=8) | 96.7-108.3 | 105.3 |
将添加有环氧合酶2(COX-2)的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
| 稀释比例 | 回收率(%) | 血清 | 血浆 | 细胞培养上清 |
|---|---|---|---|---|
| 1:2 | 范围(%) | 84.2-95.1 | 88.6-96.5 | 90.6-110.4 |
| 平均回收率(%) | 91.3 | 93.4 | 96.8 | |
| 1:4 | 范围(%) | 89.6-104.1 | 87.8-108.7 | 105.2-115.0 |
| 平均回收率(%) | 95.3 | 98.6 | 108.4 |