货号:GZEL21328
TNFAIP5; TSG-14; Pentaxin-Related Gene; Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein; Pentraxin 3; Long; alpha-induced protein 5;pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor;Pentaxin-related protein PTX3;Pentraxin 3;pentraxin 3, long;pentraxin-3;pentraxin-related gene, rapidly induced by IL-1 beta;pentraxin-related protein PTX3;PTX3;TNF alpha-induced protein 5;TNFAIP5;TSG14;TSG-14pentaxin-related gene, rapidly induced by IL-1 beta;Tumor necrosis factor alpha-induced protein 5;tumor necrosis factor, alpha-induced protein 5;Tumor necrosis factor-inducible gene 14 protein;tumor necrosis factor-inducible protein TSG-14
我司质检部门依据产品标准,在受控的实验室环境(环境温度:18-25°C,相对湿度:35-75%)和严格的实验操作(TMB 37°C避光反应194分钟后终止读数)下完成检测,确保产品性能符合要求。因具体实验环境条件及实验操作存在差异,以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
| 浓度(pg/mL) | 2000 | 1000 | 500 | 250 | 125 | 62.5 | 31.25 | 0 |
|---|---|---|---|---|---|---|---|---|
| OD值 | 2.22 | 1.56 | 0.92 | 0.63 | 0.42 | 0.25 | 0.18 | 0.08 |
分别往不同样本中添加已知浓度的五聚素3(PTX3),做回收实验,得出回收率范围和平均回收率
| 样本类型 | 范围 | 平均回收率 |
|---|---|---|
| 血清(n=8) | 84.6-101.0 | 97.1 |
| 血浆(n=8) | 91.7-105.1 | 102.7 |
| 细胞培养上清(n=8) | 96.6-108.7 | 105.5 |
将添加有五聚素3(PTX3)的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
| 稀释比例 | 回收率(%) | 血清 | 血浆 | 细胞培养上清 |
|---|---|---|---|---|
| 1:2 | 范围(%) | 84.2-95.9 | 85.0-96.5 | 90.9-110.7 |
| 平均回收率(%) | 91.5 | 93.9 | 96.5 | |
| 1:4 | 范围(%) | 89.8-103.2 | 87.6-108.9 | 105.8-115.8 |
| 平均回收率(%) | 94.6 | 100.2 | 108.4 |